Hi all
My DNA origami sample goes into a redsafe stained gel.
I seperate the sample into species/bands containing the scaffold strand, the staples and the DNA origami. All bands appear green.
If I recover the DNA origami and then subsequently run it again through electrophoresis as a 'ladder' it appears red. Everything else about its migration behaves the same. Its just now red. No post treatment. I recover directly from agarose via a freezing step/freeze and squeeze.
Has anyone else noticed this/has any kind of explanation? Im stumped.
Thanks for your time.
are you red/green colour blind?
Is the colour change verified by a woman as they are rarely red/green colour blind?
in general when complex organic molecules change colour either regions of single and double stranded carbon-carbon bonds are effected or pH effects change the colour as in litmus or other dyes
Thank you for your answer Paul Rutland. I have consulted a female researcher and unfortunately it appears i am not color blind.
Are you suggesting litmus properties in the redsafe dye? because as far as Im aware its remaining in the same TBE buffer solution throughout the procedure and through both electrophoresis steps.
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