Hi
I need to desalt a DNA sample before lyophilising it. Papers mention using a C18 column (I looked at columns and pipette tips) but when I examine the manuals available for these products they are not clear on a protocol which would be suitable for my needs (desalting and then decanting directly into a PCR tube for lypholising.
Can anyone confirm the agarose gel idea or provide more information on a tool/protocol I could use to desalt a DNA sample, with attached thiols, suspended in TCEP?
Thanks