Would I expect to see lowered CD8+ expression on T cells that are dying (Pure T cell population, majority is CD8+ at the start of a 48h-long culture)?

21 January 2017 1 103 Report

There seems to be a slight smear in CD8 staining for live cells (cells that are not stained with Live dead), whereas dead cells (cells that are stained with live dead) display an intermediate to low expression of CD8.

Valery Volk

Hi Sharon!

Good question. I went to my data to check it. Looks like - yes. I've compared in total 25 samples from different tissues (spleen, lymph nodes, blood). I was screening for MFI of CD8+ human T cells betwen Naive and Terminal effector subsets (CD45RA+/CD62L+ vs CD45RA+/ CD62L- resp.). Here terminal effector cells represent your "dying" population. And in general this subset of cells is characterized by 10-25% reduced MFI for CD8 expression comared to Naive cells. Hope it helps you.

Good luck!

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