Will I kill HUVECs when I spin/pellet them down at 700G, 4degrees?

Sharon Lee @Sharon-Lee-29

01 June 2017 0 7K Report

spun in v bottom multiwell plates

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Could you recommend some articles on Urban Transportation System optimization and Innovation?

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Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

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How prion can cause disease?

how is prion can cause disease

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Does the delta function actually make sense to use on point particles?

The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.

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How to back transform the results generated from analyses using log transformed with In(X+1) data?

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Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

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IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

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While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

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Could you try using PeptiCloud and see if it's a useful tool for biology research?

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Is there a problem with my RNA pellet?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

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Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

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How does grain and grain boundary affect the ceramic when studying its dielectric properties?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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