21 Questions 4 Answers 0 Followers
Questions related from Sebastian Freeman
Greetings everyone, Would anyone be able to suggest or know of software to model the dosage of UV received by a surface from a source object? For example, I would like to model how a cylindrical...
16 May 2020 3,342 2 View
Greetings everyone, My lab, as with others many others, have shifted some of our efforts to aid in the global fight against COVID-19. As part of our efforts, we are looking to evaluate current...
07 May 2020 564 0 View
Hello everyone, I am trying to troubleshoot my SEM procedure. I am trying to image gelatin gel microstructure using SEM. These gels extruded out of a small diameter nozzle (0.33 mm ID). I have...
31 July 2018 5,979 9 View
Hello everyone, I would like to know what buffer solution is best for maintaining bovine thrombin activity if I intended to store the thrombin solution frozen at -70 C. I do intend to use the...
13 June 2018 760 3 View
Hello everyone, I would like to infect my NIH-3T3 cells with CMV promoter lentiviral particles to induce GFP expression. The particular plasmid is from Addgene (https://www.addgene.org/17446/)....
17 July 2017 8,495 0 View
Hi everyone, When culturing a hydrogel in a well-plate, how do you decide how much medium to put over the gel? I have noticed that for 2D culture, the recommendations for medium volume give...
04 May 2017 3,076 3 View
Hello everyone, I was hoping someone might have some insight into a problem I have with filtering fibrinogen solutions. I use bovine fibrinogen from Sigma Aldrich (F8630), which is Type I-S,...
15 December 2016 2,302 5 View
Hello everyone, I am interested in immunostaining cell-laden hydrogel scaffolds for secreted proteins, such as VEGF. I can do both whole scaffold immunostaining and also cryosection. I typically...
06 October 2016 6,269 0 View
Hello everyone, I often grow endothelial cells in 3D gels, but one thing I would like to be certain of is whether or not they are developing a lumen. I have read several papers which show images...
20 September 2016 7,190 5 View
Hello everyone, I often work with hydrogels and in many cases I have small pieces of plastic tubing embedded in the hydrogel. I am not very well versed in the terminology around this subject,...
16 September 2016 8,132 3 View
Hi everyone, I have a question about accepted definitions of terminologies when it comes to hydrogels such as collagen, gelatin, alginate, etc. I have heard and read the two terms gelation and...
03 August 2016 173 3 View
Hi everyone, I have recently become interested in using the transglutaminase enzyme for the crosslinking of protein-based scaffolds. I have seen the name "Moo glue" cited in a few papers, and I...
05 May 2016 1,211 0 View
Hello everyone, I am looking in particular for MCF-7 breast cancer cells and HUVECs already transfected to express fluorescent proteins such as GFP. I would prefer the HUVECs at as a low a passage...
02 March 2016 3,863 3 View
Hello everyone, I wanted to form a solution of a mixture of rat tail collagen type I and 600 Da PEGDA. I kept everything on ice except the PEGDA, and added a little bit of phenol red into the...
26 February 2016 7,533 0 View
Hello everyone, I was particularly inspired by the paper by Antoine, Vlachos, & Rylander 2015 - Tunable collagen I hydrogels for engineered physiological tissue micro-environments. In it, they...
15 December 2015 8,123 8 View
Hello everyone, I was culturing a cancer cell line and primary HUVECs for an experiment for immunofluorescence. I had treated the plastic surface with poly-L-lysine for improved adhesion since in...
27 November 2015 7,682 6 View
Hello everyone, I have been preparing cryosectioned tissue samples of mouse and rat spleens and tumors. I followed the advice of some researchers from here to fix as soon as the tissue was...
19 November 2015 6,697 4 View
Hi everyone, I currently use a slightly modified version of Rajan et al.2006 for preparing type I collagen solutions from rat tail tendons in lab. I was wondering if anyone knew any other...
29 October 2015 9,326 0 View
Hello everyone, My lab regularly extracts collagen from rat tails of normal rats, and we would like to include steps for sterilization. I have seen some protocols but I am unsure what step or...
21 August 2015 3,126 3 View
Hi everyone, I'm interested in flow through a collagen scaffold. I am thinking of using needles to insert into the collagen scaffold hooked up to a peristaltic pump. Has anyone done this before...
18 August 2015 2,919 3 View
Hi everyone, I am designing a cell culture vessel for my experiments and I have come upon a design consideration that I am not sure how to proceed with, and that is, what would be an adequate...
01 January 1970 5,550 2 View
