How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Paired Samples t-test or repeated measure ANOVA?

Dear Research Gate members, for my masters's thesis, I have conducted a questionnaire to investigate consumer's attitude towards sport sponsorship. I've asked three identical questions...

27 January 2021 2,910 3 View

How to ensure diversity and fight stagnation in a complex two-objective optimization problem?

I am working on solving an optimization problem with two objectives by using neuroevolution. I use NEAT to evolve solutions which need to satisfy objective A and objective B. I tried different...

27 January 2021 4,875 3 View

What is the use of Carbon and Proton NMR Spectrum in Peptide Analysis?

Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...

05 January 2021 1,196 7 View

Where can I find data on the total atmospheric absorption for a standard atmosphere?

Graphs like the one attached below can be found on every website explaining the spectral properties of the atmosphere. However, I wasn't able to find the data behind these graphs. I need the...

07 December 2020 2,851 4 View

Is there some kind of Octave package (or script) available, to interact with a PEAK CAN USB (or some other CAN hardware) device?

In MATLAB, you can use the Vehicle Network Toolbox, which supports CAN interfaces. I'm searching for something similar in Octave.

06 August 2020 9,571 2 View

In Porphyrin chemistry, what are the allowed limits of deviation (In percentage) from Elemental Analysis and Mass spec?

I synthesized some (metallo) porphyrins. I am now characterizing them by Mass spec and also confirming their purity by Elemental analysis. The values I got are a little deviated from the...

26 July 2020 2,886 2 View

Can you recommend any publications or authors on "The Impact of the IT&C Sector Upon a National Economy"?

Judging by most stock markets, "Big-tech" seems to be the success story, yet there are surprisingly few academic research papers written on this phenomenon. We are looking for studies and authors...

23 July 2020 5,074 3 View

About Fortran and defining the units?

Hi to all. I am doing a large set of calculations employing as input files called 1.log, 2.log, 3.log,......, 300.log. Usually I use this definition for the input file: OPEN(5,FILE='...

12 July 2020 5,027 12 View

Submission of dna sequences to NCBI?

When i submit dna sequences to NCBI, they ask for clarification saying "Some or all of the protein coding sequences contain internal stop codons, reading frame shifts (insertions/deletions...

03 July 2020 6,101 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View