Hello everyone,

I would like to know what buffer solution is best for maintaining bovine thrombin activity if I intended to store the thrombin solution frozen at -70 C. I do intend to use the enzyme in cell culture applications, so I would also like to make sure the buffer is not toxic to cells. I have seen used a 20 mM MES, pH 6.0, 500 mM choline chloride buffer, but I am not sure whether MES is safe for use with cells. I have also seen 0.1% BSA. The data sheet for my thrombin says it was lyophilized from a solution containing glycine, sodium chloride and calcium chloride but does not indicate amounts and recommends a 0.9% sodium chloride solution for reconstitution. I am curious to know what other people use for thrombin. Thank you.