Hi everyone,
I'm interested in flow through a collagen scaffold. I am thinking of using needles to insert into the collagen scaffold hooked up to a peristaltic pump. Has anyone done this before and what have you used to secure the needles into the collagen so they don't fall out or leak? Are there any alternative methods?
Why do you want to put needles in it ? There is commercial system for the seeding of scaffolds. for instance this one :
http://www.cellecbiotek.com/default.aspx?lang=en
Hell
please , look at the Commercial products in these companies . and , the decision in RG link about { tips about seeding cells on scaffold }
https://www.bdbiosciences.com
http://www.nanofibersolutions.com/company.html)
http://www.sigmaaldrich.com/catalog/search?term=collagen+scaffold&interface=All&N=0&mode=match%20partialmax&lang=en®ion=SA&focus=product
Good Luck
https://www.researchgate.net/post/Are_there_any_tips_about_seeding_cells_on_scaffold
Hey,
I will advice you to use an ibidi perfusion system for lateral perfusion, and used cell culture 3D collagen insert mixed with your cells (Protocol N.Bursac), I think is the best solution, you just need to punch the culture gel and add it in the system. If you couple your confocal microscope with this cell culture system you can using living probs ( CDIL traker for instance) follow in real time your cells in 3D.
Best.
Philippe
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