14 Questions 2 Answers 0 Followers
Questions related from Sang Heon Kim
Hello, I want to conduct some research that a exotic plant can be an introduced plant. After I plant it in specific area, some criteria including survival rates would be estimated for a certain...
22 May 2024 3,578 0 View
Generally, in my lab, to extract RNA from plant leaf, leople grind the samples with the DEPC treated mortars and liquid nitrogen. However, it takes a long time so I want to skip this stage. Is it...
09 December 2018 681 2 View
Hello. In my lab, people use the gel-doc from the Alpha Innotech, and the AlphaImager software is intalled in the public computer. However, the OS of this computer is windows 98 SE so that it is...
02 November 2018 6,042 4 View
Hello. I am interested in salt tolerance of Triticeae crops so that nowadays I am growing some wild relatives such as Elymus dahuricus and Thinopyrum ponticum. I treated vernalization for up to...
01 November 2018 8,704 4 View
Hello. I am studying on plant breeding. In my lab, the others usually are performing researches related to molecular biology and genetics. As far as I know, general process of research is studying...
21 December 2017 9,058 2 View
Hello. I am trying to extract low molecular weight and high molecular weight glutenin subunits. There are many protocols but some of them are similar. Of them, I found two protocols. Their...
24 August 2017 1,137 1 View
Hello. Nowadays, I am studying on seed storage protein in wheat and their relatives. HMW glutenin is well-known and it has been studied a lot, but LMW glutenin has studied less than HMW glutenin...
03 May 2017 2,186 20 View
Hello. I have some questions. I am studying about seed storage protein in wheat. According to "Catalogue of Gene Symbols for Wheat (2013ed)", there are 5 loci related to Glutenin. Glu-1 is related...
06 February 2017 9,478 1 View
Hi. I want to isolate a full sequence of a HKT (High affinity K+ Transporter) gene from unknown plant species. According to some papers, Xenopus oocytes were used to confirm the gene but I don't...
16 January 2017 6,305 2 View
Hello,I am planning to compare the stress tolerances such as cold, heat, or salt (not determined yet) of some crops and their relatives.They are classified as same tribe but not same genus.There...
01 December 2016 3,148 3 View
Hi. Nowadays, I am interested in the gene whose sequence and function was verified from some crops such as wheat and rice. I want to get the full sequence of the gene from a wild relative of...
05 November 2016 6,176 3 View
According to many studies, seedlings were treated at the 3rd leaf stage. Why at 3rd leaf stage not 1-2 leaf stages or 4-5 leaf stage? I am looking for the reference but it couldn't be found.
14 May 2016 6,712 1 View
When performing genetic research on the species which is never studied, there is no information about housekeeping genes. In this case, first after I obtain the sequence of the candidate...
01 January 1970 6,494 2 View
Hello. I am trying to secure Actin sequences from plant species which have not yet registered in NCBI and to develop species-specific sequence characterized amplified region (SCAR) markers to...
01 January 1970 814 0 View
