Hello,
I am planning to compare the stress tolerances such as cold, heat, or salt (not determined yet) of some crops and their relatives.
They are classified as same tribe but not same genus.
There are many studies about stress tolerance in one species that used accessions under stress to compare relatively tolerant accessions and susceptible accessions. However, in the study that I am planning, the problem is I don’t know how many accessions are significant to represent the species or the genus.
It is impossible to test all of accessions in the world. However, only one accession can’t represent the whole species. Then, at least, how many accessions should be used in each species when comparing different species?
you can go with the match having highest % similarity and also go for NGS further for getting a closer profile of your organism .
Statistically, a safe way is to get a degrees of freedom, which is equal to or more than 21.
I can give you link of our a very recent article:
http://journals.tubitak.gov.tr/agriculture/issues/tar-16-40-5/tar-40-5-13-1604-59.pdf
Hope it would be helpful.
Please ask if you have further question.
Best regards.
NusretZencirci
Better to go for more no. of accessions of one species while comparing with other species to minimize the statistical error. It depends on the availability of accessions of any species. Particularly for cultivated species more no. of accessions are available, but for wild species it is not.
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