Why was treatment performed at 3rd leaf stage?

Sang Heon Kim @Sang-Heon-Kim

14 May 2016 1 7K Report

According to many studies, seedlings were treated at the 3rd leaf stage.

Why at 3rd leaf stage not 1-2 leaf stages or 4-5 leaf stage? I am looking for the reference but it couldn't be found.

Sang Heon Kim

Thank you for your answer. Then, how about in monocot plants?

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