According to many studies, seedlings were treated at the 3rd leaf stage.
Why at 3rd leaf stage not 1-2 leaf stages or 4-5 leaf stage? I am looking for the reference but it couldn't be found.
Thank you for your answer. Then, how about in monocot plants?
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A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.
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