Hi.
Nowadays, I am interested in the gene whose sequence and function was verified from some crops such as wheat and rice. I want to get the full sequence of the gene from a wild relative of wheat.
There is no database in this plant, so I designed the primer based on the conserved site in the wheat and other crops.
Soon, I may get the full sequence of the gene from my plant.
I will carry out qRT-PCR with the primer designed from the sequence I obtained and investigate the expression level before and after the treatment. Also, I will compare the sequence from my plant and that of wheat or other crops.
Is it enough to publish the article? Normally, people isolate some genes from new species, they transformed the gene into Arabidopsis, Yeast, or other plant. Can I publish the article to SCI or SCI-E journals without transformation experiments?
If you want to say that the gene encodes a protein that has the same function it best if you transform a knock-out line but it does not have to be plant. For instance depending on the function of your gene, if it has been well characterized in yeast, or drosophila you could complement a ko in those organism to show that the function is conserved. Is that clear ?
Without transformation data, look like you have only two results (from your description): (1) the sequence data (of the gene, and the comparison of sequence of the gene from other species), (2) the gene expression profile of your plant species. Personally, I don't think this is enough to vouch you a SCI paper with better impact factor. I agree with Hugo, reviewers would be appreciated to see some 'biological function' data of this gene. Especially the gene function is known in other plant species.
you need to do functional analysis, better transfer the gene into Arabidopsis and characterize the gene function.
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