The average plasma concentration of testosterone in male rat is difficult to determine because it significantly varies depending of stress and age of the animal. I suggest you to read this article (http://authors.elsevier.com/a/1R1aT3QIyTrDwi) where are grouped (among others) the various steroid measurements in young and middle-aged rats.
we have measured serum and testicular concentrations of both testosterone and 17 beta-estradiol in male Wistar rats. You can find more information in this publication.
Kind Regards,
Luís Pedro Rato
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There is a big problem with the results of RIA estimations of estradiol in rats. As we published earlier, direct estimation of estradiol in non-chromatographed extracts of rat serum yield results, which are much higher than those in samples after chromatography on LH-20 columns, presumably because of unknown compounds of adrenal origin ( de Jong et al, J. Endocrinol 1973; 57: 277-284). The level of estradiol in peripheral plasma we described (7.3 pmol/l) is in line with the level in testicular venous plasma (64.3 pmol/l), if 20% of circulating estradiol in male rats is of direct testicular origin, as is the case in men.
We also measured levels of estradiol in serum of male rats 20-24 h after the last of 7 daily injections with various doses of estradiol in oil. Daily injections with 1 microgram estradiol lead to concentrations of around 200 pmol/l; 10 or 100 microgram/day yielded results which were 10- or 100-fold higher. Effects of tehse injections on testosterone levels were only obtained with the two higher doses, which also suppressed LH to undetectable levels.
Measurements of testosterone in male rat plasma are less problematic, and should yield concentrations in the order of 8-15 nmol/l.
Hi "Total testosterone plasma concentrations of male rat (24 week old) 3.54± 1.01 ng/ml, Male-Cast 0.17 ± 0.02 ng/ml (Page 272- Table 3)" You may want check this article : Mor, F., Kilic, M. A., Ozmen, O., Yilmaz, M., Eker, I., & Uran, K. (2014). The effects of orchidectomy on toxicological responses to dietary ochratoxin A in Wistar rats. Experimental and Toxicologic Pathology, 66(5-6), 267-275.