02 October 2016 0 498 Report

is there a method for measurmnet of lactate and pyruvate on heart homogenate

More Regouat Nadia's questions See All
Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Could you please guide me how can I manually separate genomic and plasmid DNA from algae simultaneously?

Next step is doing qPCR. Thanks

23 April 2024 5,442 5 View

Hi, I am going to isolate genomic and plasmid DNA from algea simultaneously and manually, Do you have any suggestion?

I found a kit that they separated plasmid and genomic DNA form mammalian cell, but I want to extract both plasmid and genomic DNA manually. I also want to use the extracted DNA for qPCR.

23 April 2024 1,857 1 View

Welche Effekt hat die Diabetesedukation durch Krankenschwestern bei Diabetes Typ 2 Patienten in Primärversorgungszentren?

„Welche Effekt hat die Edukation von strukturierte Schulungsprogramme, wie Disease-Management-Programme (DMPs), durch geschultes Pflegepersonal auf den glykosyliertes Hämoglobin-Wert (HbA1c) und...

14 April 2024 248 2 View

Do you have references dedicated to the influence of batch for the same steel with the same process and the same composition?

I am trying to find papers dedicated to the effect of a change of supplier or batch on the variation in the tensile strength of the steel.thank you

04 April 2024 1,897 0 View

What is the impact of external predisposing factors on the oral health status of cerebral palsy patients?

how the caregiver influences the oral health status of a child with cerebral palsy

22 February 2024 395 1 View

Why ions are not detectable on a bare electrode?

Why metal ions with high half potential can't be detected on a bare electrode even though corrosion doesn't happen and I'm using the suitable carbon working electrode and Ag/AgCl for the reference...

31 January 2024 333 1 View

What is The Impact of GPt on Educational supervision ?

Why researchers must be cautious about using ChatGPT in academic research? Does using ChatGPt have negative influence on researchers skills? what researchers' skills are negatively influenced...

05 January 2024 6,680 0 View

WR99210 from medchem express ?

I am planning to use WR99210 from medchem express for drug selection of the mutant parasite. does someone know if this WR from medchem express has been tested in Plasmodium parasites ?

14 November 2023 8,339 0 View

How to Simulate Symmetrical Tapered Waveguide Model Using COMSOL?

Hi, Anyone use COMSOL to simulate Symmetrical Tapered Waveguide Model? I have followed Tapered Waveguide model in COMSOL Application, but the structure is only tapered down area. I have add...

14 August 2023 8,580 0 View

Similar questions and discussions
Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How does the melting point of a spherulite correlate with its size?

For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...

30 June 2024 2,891 1 View

His column crack?

Hi... I seem to be in a very big trouble. My His columns look cracked after my last purification. In another column, the materials seem to be pushed. How is it possible and is there anything I...

24 June 2024 7,071 2 View

Inquiry Regarding Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT)?

Dear All, I am writing to solicit your expertise regarding the application of Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT) for structural...

22 June 2024 7,751 5 View

Replacement for acetone in dehydratization of cryo sections?

We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...

10 June 2024 5,213 3 View

Culturing muscle cells in DMEM?

Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...

28 May 2024 1,398 0 View

Why Hela cell (ATCC CCL-2) didn't adhere?

Hello, We recently seeded a HeLa cell line purchased from ATCC, which was received over six months ago (stored at LN2). We used the following media composition: DMEM supplemented with 10% FBS, 1%...

27 May 2024 7,312 2 View

How do I obtain sturdy hydrogel that doesn't dissolve in water upon centrifugation?

I have prepared cellulose nanofibers (CNFs) using TEMPO oxidation method. After sonication, the thin, heterogenous suspension turns into a thick, homogenous gel-like solution. Upon first...

13 May 2024 2,413 1 View

Could anyone help me with my mouse CD3+ T cell culture?

Hi everyone, I'm going to generate CAR-T cells starting with mouse CD3+ T cells. I isolated mouse CD3+ T cells using Miltenyi Pan T cell isolation kit. I seeded cells 1millon/well in 24 well...

07 May 2024 6,908 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close