Why does kaleidoscope Marker miss Bands in the middle??

We had several times the problem after the transfer: you can detect the yellow 10 kDa Band, the blue 20 kDa Band, the pink 25 kDa Band but after only the pink 75 kDa Band. 15, 37 and 50 kDa are...

02 February 2021 4,936 3 View

What's difference between NH4+ and NH4+-N, NO2-and NO2-N, NO3- and NO3-N?

Hello, Upon writing my paper, I noticed that most journal papers stated the concentration of all nitrogen compounds in -N , example instead of NH4+ its NH4+-N. I knew that the -N form uses the...

14 December 2020 3,693 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

No title

I have an AlN sample and the EDX data show me the at% of a single atom such as Al, N and O . In order to measure the at% AlN , I have to use the chemical formula 2AlN = 2Al + N 2 . should I use...

20 August 2020 6,269 3 View

What is the impact of solder mask and Surface finish on antenna performance?

Hello everyone, I am working on the design and fabrication of a butler matrix connected to an array antenna. The structure operates at 3GHz. In fact, during the design, I did not include any...

31 May 2020 1,873 2 View

How to simulate a bio inspired Artificial Optimization algorithm based geocast routing for VANET?

I would like to simulate a bio-inspired optimization based Geocast routing for VANET using a clustering algorithm in ns3 or Omnet++ with SUMO. And , evaluate my approach with other existing...

23 February 2020 8,108 4 View

Looking for protocol: mitochondria isolation from treated cell line with subsequent Analysis in Seahorse XFP (8well)?

Hey guys, i would like to know, if there is any existing protocol, how to isolate mitochondira from treated cells and use them further in Seahorse XFP Mito Stress test for 8well plates? Second...

29 October 2019 7,474 1 View

How may I clone a 5415 bp gene into pglo plasmid (5371 bp) replacing it's GFP gene (~700 bp)?

I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...

05 October 2019 4,938 7 View

Wich is the post-hoc test for Conway-Maxwell-Poisson (COM-Poisson) distribution?

I used the Conway-Maxwell-Poisson distribution for my underdispersed count data. Now I'm interested in compare the treatment but I don't found the script for it. For the others dsitributions...

10 September 2019 6,835 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View