Hi, you have to proceed with titering at 1:100, 1:200, 1:400, 1:1000 with a standard secondary antibody.  The method is trial and error,  just save some tissue for this!  

Good luck 🍀 

It depends upon the samples, since you can make the cocktail of the antibody mixture and then add to each sample. Secondly if its conguate like PerCpcy, Apc etc then it works normally good at low concentraions as compared to FITC etc. Like if you have 20 Samples to be stained means 20 tubes , you can use 5microL PercpCy, but you have to increase the volume of FITC to be used....