Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?. I need a valuable suggestions so Please help me. Thanks!
Some strains are more difficult than others to transform, however, if your strain does not carry a resistance that is similar to the one carried on the plasmid then you can pretty much pick what you want.
Hello, Transformation of E. coli using green fluorescent protein.
see link below may be useful for you
https://www.apsnet.org/edcenter/disimpactmngmnt/labexercises/PlantBiotechnology/Pages/Activity4.aspx
good luck!
As mentioned by Hanna Alalam it may depend a bit on the strain but in theory you should be able to transform nearly any E. coli strain. However whether you get good expression of the GFP will depend upon the construct itself, is there a suitable promoter and appropriate translation signals for GFP expression in E. coli.
Traci L Testerman Michael J. Benedik Karen A. Darbinyan Hanna Alalam Thanks a lot for your helpful inputs as it will easy for me to do regarding my lab procedure.
Yes the transformed cells can be visualized as fluorescent colonies under a fluorescent microscope since E. coli does express GFP properly.
Ramiz Raza
Make sure the gfp gene has no intron in it. Some have an intron build in it, to make sure the gene not to express in prokaryotes, but in eukaryotes. And, make sure the promoter can work in E. Coli.
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