Does different microscope measure different width(e.g. x, y and z rather than x only) of a particular device having only x width at the same objective?
Do you mean the distance in XY axis? There cannot be just X or Y as it becomes a point not the distance. The distance is the distance from point X to point Y.
If I understand your question right: The measurement on the scale bar varies depending on various factors -
- The objective magnification
- The NA of the objective
- Camera pixel size
- Resolution
- Other factors include the components inbetween the image to the final image that you see (like the tube lens, condenser lens, etc.)
All the above factors varies from one microscope to other until unless they are exactly the same (similar camera, objectives, NA, etc.). The Z resolution (Point Spread Function) is around three times worst than the XY resolution and the resolution is determined by the NA of the objective besides other factors.
Check the following link about magnification:
The Concept of Magnification -
https://www.olympus-lifescience.com/en/microscope-resource/primer/anatomy/magnification/#:~:text=The%20image%20is%20a%20virtual,observation%20eyepiece%20of%20the%20microscope.
Good luck!
@Sathya Srinivasan
Sathya Srinivasan Dear Sathya, I was asking about width of the device in the form of x, y and z (you can simply assume either number 1, 2 and 3 or a, b and c in place of x, y and z), I am not saying about XYZ-axis ( sorry for using x,y and z format as XYZ-axis). Though, You have suggested and explained a very well manner of various factor that governing microscopic image measurement. Thanks for all this things and sharing of link regarding image measurement.
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