Petri dishes are incubated in inverted position i.e the plate lid face down and the bottom of the agar face up in order to reduce rate of evaporation which results in proper microbial growth,
Yes they should be face down. The main reason is that when face up you can get fluid condensation (puddles) on the plate that will interfere with getting distinct colonies. This is reduced when face down.
petri dishes for microbiological analysis should be incubated in inverted position those improve the result accuracy.
When the plates are incubated in the normal position, water evaporation from media occurs. These vapors condense on the lid of the Petri dish and drops fall on the colonies developed on the media surface. This causes colony mix-up with each other and spread throughout the plate surface. This creates the problem in counting and proper determination of microbial count.