Is it acceptable to add more conclusions in a conference paper that were not discussed due to shortage of space?

Hi, My research project had numerous results and because the conference paper is usually just 5-6 pages long, I could not discuss all my results in it. However, is it still possible to mention...

04 May 2020 527 5 View

How long will it take to digest the food in the absence of digestive enzymes in our body?

We all know that digestive enzymes speed up the chemical reaction and helps in food digestion. I was wondering if in general it takes 6 h to digest a food with the help of digestive enzymes then...

04 October 2019 5,419 3 View

Can anyone suggest me a better coating reagent other than Poly-L-Lysine for the adhesion of HEK-293T cells?

I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...

24 May 2019 281 2 View

How to perform cell based assay in 96-well plate using HEK-293 cells as the cells shows poor adhesion and comes off quicking ?

I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....

01 May 2019 3,563 9 View

Does all enzymes are synthesised by ribosomes in zymogen form or are there expections when enzymes are synthesized in active form?

Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?

17 March 2019 4,341 3 View

Is it possible to obtain a dimer in SDS-PAGE reducing gel?

I have purified a monomeric EGFP- His protein with a molecular weight of 30 kDa. But, instead of 30 kDa I am getting a thick band of 60 kDa in SDS-PAGE reducing gel. what is the possible reason...

30 January 2019 5,719 2 View

Why the Ni-NTA purified His-tagged EGFP protein is coming at 60 kDa instead of 30kDa on SDS-PAGE gel?

I have purified a EGFP-His tagged protein which was there in pET15b vector using Ni-NTA column. The concentration obtained was way too high around 5mg/ml. Before the running the SDS-PAGE the...

30 January 2019 7,231 2 View

I have to perform FRET assay using a microplate reader in a 96 well plate. Please suggest me some good literature to perform the assay?

Have to see protein-protein interaction in HEK-293 cells, where the donor GFP tagged protein and acceptor is RFP tagged protein. Please suggest me some good tips that I should keep in mind for...

11 January 2019 7,021 1 View

Which is the best storage buffer for the recombinantly purified mEGFP-His tagged protein ?

Best buffer composition for storing a recombinantly expressed and purified mEGFP protein ?

11 January 2019 8,647 4 View

I have to purify a GFP-His tagged protein. Kindly suggest me some good literature in this regard ?

I have a vector pET23b with monomeric EGFP-6X His tagged cloned in that. The plasmid is transformed in BL21(DE3) cells. Kindly suggest me some good literature for the purification of the GFP-His...

11 January 2019 2,368 4 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View