FLAG tag the octapeptide sequence and artificial epitope binds to the FLAG antibody. I want to know in which region of the FLAG antibody does the FLAG epitope binds ?
Antibodies bind their epitopes in the complementarity determining region, which is within the Fab part of the molecule.
Hi Puneeta, I have heard from many researchers that the FLAG-tag purification is very expensive and not worth the cost.
any scientific method?
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