Naresh Niranjan Dhanasekar

13 Questions 10 Answers 0 Followers

Questions related from Naresh Niranjan Dhanasekar

Lipid molecule charge?

I am unsure whether the charge of this lipid negative or neutral. I know PE alone is negative but not sure whether Egg Liss Rhod PE is? Thanks https://avantilipids.com/product/810146

05 June 2024 4,812 1 View

Making glass slide/coverslip hydrophobic?

Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...

22 May 2024 9,331 3 View

Question about Lipid charge?

I was wondering what is the charge of this lipid? Is this neutral or positively charged? Thanks for your answer? 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl)...

22 May 2024 4,745 0 View

How to make a homogenous droplets using mineral oil and water?

Hi, I am currently involved in making water-oil droplets, and I am having issues getting the droplets. So basically, I use light mineral oil and water in the ratio of 8:2. I do not use any...

04 March 2024 6,375 5 View

Lower current for larger diameter DNA nanopores?

I have been working on a DNA channel which has an internal diameter of about 10 nm, and a length of about 60 nm. However, the conductance what i observe in single channel electrophysiology is...

30 October 2018 2,872 1 View

Issue in droplet interface bilayer technique?

As soon as the bilayer is formed in DIB the current level increases and it does not come to zero, it always gives a current about 400 pA @ zero voltage. I really dont understand why? Further when...

16 November 2017 2,112 0 View

How to confirm the presence of bilayer using the droplet interface bilayer technique?

Is it necessary to send a signal of 10Hz (frequency) with an amplitude of 500mV peakto peak to get a triangular wave form for the formation of DIB bilayers. Further, is it necessary to use the...

30 October 2017 2,933 2 View

Hi which mode should i use in the nanodrop to measure the DNA origami concentration. Whether it is DNA-50 or SSDNA-33?. Thanks

DNA nanotechnologist

12 July 2017 8,143 2 View

Could anyone explain the mechanism behind the insertion of DNA insertions into lipid bilayers. Does this pattern follow the same as membrane protein??

Membrane biologist Nanopore

31 March 2017 5,955 2 View

Could anyone help me in combining three or four ion current traces into single trace using clampfit software?

12 July 2015 1,868 3 View

How do you determine the net charge of a peptide, i.e. triargine?

see above

18 March 2014 1,445 5 View

What could be a possible explanation for why porin always inserts into the artificial membrane when negative voltage is applied?

see above

05 March 2014 1,373 2 View

How to predict the structure of the outer membrane protein through homology modelling?

I am planning to work on the outer membrane protein of a bacterium, which leads to gastroentritis. I found out the best way to target is through the outer membrane protein.

17 September 2012 251 1 View