A possible explanation is that you have a porin that has a global charge (negative if you have the ground at trans):
If you have the ground at trans, negative voltage imply higher electric potential at the trans side. In those experimental conditions a negative charge added at cis will move toward the side with higher potential, the trans side, by the effect of the applied potential.
As a consequence, the negative applied potential will speed up the channel insertion into the lipid bilayer.
Many thanks for the response Prof. Lidon.
Is this condition is true when you add the protein on the trans side where the live electrode is present, i mean where I apply the voltage and subsequently it requires positive potential to pull the protein to the bilyaer am i right?
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