Dear Naresh Niranjan Dhanasekar

First of all, PE has (at physiological pH) a neutral charge. See for example https://www.phospholipid-research-center.com/phospholipid/types/ although the Lissamine Rhodamine B Sulfonyl looks quite complicated I automatically assume that this labeling does not alter the overall charge (otherwise one would induce a positive or negatively charged phospholipid in your assay while phosphatidyl ethanolamine is zwitterionic/neutral).

Best regards.

PS. It is for a reason that most head group labelled phospholipids are neutral ones (PC, PE) https://avantilipids.com/product-category/fluorescent-lipids/phospholipids-fluorescent-lipids/headgroup-labeled

As you might know the head group of phospholipids is quite often a determining factor in various processes:

Article Effect of the combination of cationic lipid and phospholipid...

Chapter Lipid involvement in protein translocation