Which column from GenePix sheet correlates to the color in the cluster diagram?

25 February 2020 3,703 1 View

Is there a synthetic DNA modification that could prevent extension of the opposite strand?

For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...

27 January 2020 9,085 3 View

Where in M&M 1913 article is it stated that [E]0 << [S]0?

I've been reading the Johnson & Goody translation of "Die Kinetik der Invertinwirkung" and I can't find the part where authors (M&M) actually claim that [E]0

15 March 2017 3,836 4 View

Can I analyze the results of this experiment with t test?

I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...

09 December 2016 4,440 16 View

Can I model a composite plate in a Nastran/Patran program so the result will give stress values at a specific thickness?

In modeling composite plates in a Nastran/Patran program using shell elements, results in the output file are for the mid-plane of each layer of composite. Does anybody know if it can be possible...

24 August 2014 1,725 6 View

How I can separate, purify, isolate BSA from DEX BSA?

I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.

21 August 2014 4,896 1 View

Can anyone explain the differences between programs for designing primers?

Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...

31 July 2014 2,648 1 View

Can anyone give me their advice on changing PCR settings for annealing and/concentration in Real Time Sybr green PCR?

I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...

07 July 2014 3,890 3 View

What is the difference between heparin, citrate and EDTA tubes for blood collection and what is the effect on DNA?

Advantages/disadvantages, troubleshooting when doing real time PCR?

27 April 2014 8,274 9 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

How to calculate cross correlation of two signals from the same source one of which is going through a filter ?

A signal is split into two parts and one of them is going through a filter (say, with a transfer function H(f)) and the other part stays unchanged. Then I want to know how to calculate their cross...

01 February 2021 5,101 8 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Matrix based scientific data analysis: extrapolating to matrices of sets?

Data sets, when structured, can be put in vector form (v(1)...v(n)), adding time dependency it's v(i, t) for i=1...n and t=1...T. Then we have a matrix of terms v(i, j)... Matrices are important,...

30 January 2021 9,295 6 View