29 Questions 13 Answers 0 Followers
Questions related from Miguel Pereira-Silva
What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?
05 July 2024 6,871 2 View
If you prepare drug-loaded nanoparticles but only obtain microparticles (~2,000-4,000 nm), PDI~0.2, how can you reduce the size to reach ~100 nm?
03 June 2024 2,635 4 View
How to know if two drug molecules interact with each other, the strength (and type) of interaction and the possibility to form nanoparticles?
03 June 2024 4,243 1 View
Curious to know your opinion on any characterization technique or step that should be looked into more detail & more often covered in order to increase the quality and robustness of...
17 May 2024 7,234 2 View
How do you wash your dialysis membrane for drug release studies?
31 May 2023 6,218 0 View
What does "nanoprecipitation" mean regarding nanoparticle formation?
03 May 2023 4,279 3 View
Several nanoparticles are self-assembled structures, such as micelles. What about PLGA nanoparticles?
03 May 2023 9,608 1 View
How to maximize encapsulation efficiency of a drug in cyclodextrins, particularly B-CD and Y-CD?
17 March 2023 760 3 View
Blank micelles formulation of surfactant X are transparent, however when preparing the correspondent drug-loaded micelles through solvent evaporation/nanoprecipitation method, the medium turns...
08 March 2023 9,930 3 View
How to maximize encapsulation efficiency of a drug in micelles?
08 March 2023 7,355 2 View
I would like to know your opinion on producing more monodisperse micelle populations and also tricks to avoid size variability and improve the reproducibility of the manufacturing process?
21 February 2023 8,446 3 View
Increasing unimer concentration for micelles preparation leads to increase of micelle size or increase in number of micelles? What are the mechanisms that dictate the behavior of the micelle...
16 November 2022 2,617 4 View
Several methods have been reported for micelle preparation. What do you think is the best one? Thin film hydration method? Or dropwise addition to water method? What are the possible advantages...
02 November 2022 1,432 4 View
I would like to connect with researchers working with cell membrane camouflaged/cloaked/coated NPs!
27 October 2022 5,446 0 View
Prodrug nanosystems with amide bonds can be possibly prepared for enzyme-responsive drug release. What do you think? In which conditions? Why and how?
06 October 2022 6,713 1 View
I would like to know the influence of pH on polymer/surfactant abilities to self-assemble into micelles.
02 February 2022 7,573 4 View
I would like to know your opinion on surfactant charge and self-assembly into micelles.
02 February 2022 6,937 1 View
Typically, adding salt to the medium is known to decrease CMC and thus favor micellization. However, what is the mechanism behind it? What are the differences between an ionic surfactant/polymer...
02 February 2022 4,194 4 View
Some diseases can have specific membrane proteins as targeting markers that endow biomimetic nanoparticles with natural tropism to affected areas, such as CD138 in multiple myeloma. Which examples...
17 January 2022 9,808 3 View
I would like to know your experience on the potential impact of the extent of dilution of a micelle formulation on the variability of Zetasizer parameters (surface charge, PDI and size). Thanks
17 January 2022 4,495 5 View
I would like to know your opinion on the eventual role of hydrophobicity/hydrophilicity of a nanocarrier surface on cellular uptake and the mechanisms imparting differential cellular uptake.
15 December 2021 9,789 4 View
What have been your experience regarding factors that influence micelle formation via self-assembly prepared by dropwise addition? For example, what would be the preferred addition speed for...
10 September 2021 3,807 4 View
Hi! I'm currently optimizing the coating of polymeric nanoparticle cores with biomimetic cell membranes. However, I would like to know general opinions on the coating mechanism of nanoparticle...
11 June 2021 2,599 0 View
I'm preparing enzyme-responsive polymeric nanosystems and according to the manufacturer's information, the enzyme is in -» "50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0."; -»"≥10 units/mg...
01 June 2021 9,779 4 View
Why do some standard formulas for PBS buffer preparation incorporate KH2PO4 and Na2HPO4 as acid-base pairs instead of KH2PO4 / K2HPO4 or NaH2PO4 / NaHPO4?
11 May 2021 1,824 4 View
Both additive and synergistic activities have been described for chemotherapy drug combinations co-loaded in the same nanoparticle. Such strategies have been aimed at cancer therapy via dual...
06 October 2020 5,291 5 View
The main (and generalistic) advantages and disadvantages of each type of nanosystem have been widely pointed out in literature. However, the advantages and disadvantages stated are too broad and...
24 June 2020 4,311 1 View
Nano-based approaches for cancer therapeutics are undoubtedly important to tackle cancer. However, it is widely known that each cancer type has its own peculiarities and heterogeneity. This way,...
01 April 2020 6,659 3 View
Which areas will develop further and gain momentum? Which ones will be showing less interest? Major targets? Major hopes? Which bounderies need to be overtaken? Which ones will remain...
13 April 2019 10,153 6 View
