27 Questions 46 Answers 0 Followers
Questions related from Meriem Fassatoui
Hello , I'm asking you why they consider that Wilcoxon test and U Mann Whitney test are the same ? Can we use this test to examine 2 numeric variables for independent groups ? Thank you
09 September 2018 8,682 3 View
Hello, I am conducting a research on the key domains of a different protein sequences. Can you give me the link of the best software that I can use to do this task ?
12 December 2017 5,806 3 View
hello, i want to know why according to the literature when they establish standard curves they use cloned bacterial DNA (for exemple for feacalibacterium strains) in a plasmid and for other...
05 May 2017 1,197 2 View
Hello, I want to compare data related to nutrition of people to standard ones in order to see if diets are hyper or hypo caloric...Can you help me in order to find standard norms of nutrition...
12 December 2016 1,269 0 View
Hello, I want to sequence rRNA16S of bacteria by means of next generation sequencing technic using Illumina MiSeq technology. In articles i see that they choose hypervariable region V4 or V6...
12 December 2016 1,514 1 View
Hello, I have 500 SNPS (single nucleotide polymorphisms) of cases and controls individuals, if i will use snpassoc (tool r studio) it will take too much time to insert variants, what is the best...
08 August 2016 6,183 1 View
Hello, I want to know when can we use Student's test, Pearson's correlation coefficient test and Spearman rank correlation, what can i put us matrix (numeric variables as means..sd (standard...
05 May 2016 4,505 7 View
Hello, I conserved the extracted microbial DNA from kit in -20°C (in TE BUFFER), after that i was obliged to transfer the microbial DNA to + 4°C because i can't ensure the transport from a place...
04 April 2016 6,885 3 View
Hello, For the SNP rs5219, i looked for the GMAF, i found T=0.2630/1317 but HGVS names indicated NC_000011.10:g.17388025T>C, What can i do in this case, there are some contradictions.? Thanks
03 March 2016 8,619 1 View
Hello, I need the definitions and utilities of each biostatistics tests quoted and the tutorial (tool r/matrix models) in order to achieve that. I need also the definitions and utilities of each...
11 November 2015 8,496 0 View
Hello, Genotyping can be proceeded by Affymetrix...chips. Who can tell me which one is more performant? What are the differences between those chips ? Thanks
07 July 2015 4,013 0 View
Hello, I want to know which formula can i applicate to calculate "the power analysis " of a scientific project, how many patients should be recruted (cohorts) in order to detect linkage with a...
06 June 2015 2,996 7 View
Hello, I must cultivate strictly anaerobic strains to extract DNA after, except that the culture method is not recommended given the time it will take to conduct this experiment I want to order...
04 April 2015 1,468 5 View
Hello, In order to conduct QPCR (TAQMAN method) manipulations I will need bioinformatics tools to check the specificity of the probes and primers for selected strains. what these letters refer to...
04 April 2015 7,513 7 View
I see my colleagues pending the arrival of the command they work with different outdated restriction enzymes in many reactions when I ask them to stop these reactions because we must respect the...
03 March 2015 850 12 View
I found in articles primers specific to different strains eg: Akkermancia muciniphila ,Bacteroides vulgatus... I noticed that many people still check these primers on blast to ensure their...
02 February 2015 5,990 4 View
When can we use them ? Why after a multiplex PCR sequencing is not carried ?
10 October 2014 5,618 6 View
Who can tell me what these letters refer to, it's frequently used in the primer sequences designated universal 16 S (R-M-K-N-W...........) ? For example: 27F (AGAGTTTGATCMTGGCTCAG) Thanks
09 September 2014 6,756 7 View
Who can help by answering the question i asked ? Why DNA cloning is not necessary using pyrosequencing approach ? Thanks
09 September 2014 4,920 10 View
Who can tell me which Mycoplasma strains may be involved in human cancer?
08 August 2014 912 6 View
I have to construct a genomic library (bacterial DNA) by using the technique GLPCR (Genomic Library PCR) who can guide me? Shall I digest DNA and vector (plasmid) by various enzymes? I would like...
08 August 2014 8,696 7 View
I extracted DNA from bacteria by classical approach or protocol phenol/chloroform method. Is it possible that this DNA is inactivated after extraction? If yes, which is the component that has...
07 July 2014 8,186 15 View
I have to test mycoplasmas in cell cultures, and they asked me to prepare a medium cmm Culture. I do not have the protocol to prepare this medium cmm.
04 April 2014 2,990 0 View
I am leading a cloning using pBluescript vector. When I arrived at the ligation step I realized that it involves specific working conditions applied in the case where cohesive ends are implicated...
04 April 2014 1,532 1 View
I plan to clone a bacterial gene. I ordered the plasmid vector pGEX but I just learned that the order will arrive in six months. Who can offer me a protocol so that I can build a cloning vector...
04 April 2014 9,827 9 View
04 April 2014 8,352 7 View
I looked for the Enterococcus faecalis strain ATCC 29219 in http://www.straininfo.net/advancedSearch.action and I found nothing. I want the record identification of the strain in question, can...
04 April 2014 5,229 8 View
