Can Streptococcus pyogenes and viridans be grown & cultured on Brain Heart Infusion (BHI) Agar for antibiotic sensitivity testing?
Dear Sony.
According to the rules of CLSA sensitivity testing of streptococci (including viridans and pyogenes) to antibiotics must be performed on Mueller-Hinton Agar with 5% sheep blood. These organisms grow very well on the BHIA, but to the sensitivity testing BHIA is not suitable. Only for culturing and growth.
I have cultured pyogenes on TSA and can usually passage the organism once before it stops growing. Although BHI is a richer media I would stick to blood agar especially for a antibiotic test.
Yes, it is possible to grow pyogenes and viridans in Brain Heart Infusion agar for your purpose.
They grow well in BHI agar. Todd Hewitt broth is basically BHI broth
Dear Sony.
According to the rules of CLSA sensitivity testing of streptococci (including viridans and pyogenes) to antibiotics must be performed on Mueller-Hinton Agar with 5% sheep blood. These organisms grow very well on the BHIA, but to the sensitivity testing BHIA is not suitable. Only for culturing and growth.
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