This will give you a population of ~10ˆ7, which is quite high for susceptibility tests. Check CLSI Methods (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically M07-A8) if you want to do this test properly. If you're working with anaerobes, check their method M11-A8.
This will give you a population of ~10ˆ7, which is quite high for susceptibility tests. Check CLSI Methods (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically M07-A8) if you want to do this test properly. If you're working with anaerobes, check their method M11-A8.
Dear Mac, as Fernando suggestion you should follow CLSI or EUCAST instructions for antimicrobial testing. For homogeneous distribution of bacteria on the surface of plates, we use sterile swaps. The bacteria prepared in McFarland 0.5 concentration inoculated by swap on the surface of mueller hinton agar plates.
I have proposed the pour-plate technique, in which the 0.5 McFarland inoculum will be mixed with molten agar so that bacteria will grow on all areas of agar after solidification.
In my experience, pour plate distributes the organism better than swabbing or spreading. Bacteria grows in the entire agar medium from both inwards & outwards. Pour plates look more quality than others.
During my research work, I observed pour plate techniques show submerged colonies. It is preferable to use swabbing or spreading uniformly and 0. 1 ml should work.