To avoid any chance of contamination and if there is availability in presence of other incubators,I said no.At least fungi, bacteria in two separate incubators.Within bacteria,you may incubate sporulating bacteria alone in small incubator.
I said NO. Cross contamination can be occurred due to all plates incubate in same incubator. I agreed with Dr.Gamal Enan answer.
Yes. It's safe unless you are not cleaning the inside of the incubator or using highly contagious ones or Petri plates without lids.
Bacteria will not go and contaminate by themselves unless an insect/mite/object does help. That's why cleaning inside the incubator is vital.
In the practice, all plates are actually stacked one above another safely inside the incubator in an inverted position along with the kids closed.
BACTERIA CANNOT JUMP, BUT YOUR INSTRUMENTS MAY JUMP FOR IMPROPER HANDLING!
Yes you can always use the same incubator for incubating plates with different bacterial cultures.
You can use parafilm to cover the lid or aluminium foil to cover the whole plate if you want.
Usually it is safe to incubate different bacterial culture plates in one incubator at the same time. But mishandling may cause cross-contamination. To reduce cross-contamination, I agree with Varsha. In our lab, we usually use parafilm to seal the bacterial culture plates or use a petri dish bag to store the bacterial culture plates in the incubator.
It is safe,
1. if it is not too congested
2. If there is no fungal plates there
3. If plates containing motile organisms are not made to have too close contact and the media not beyond normal level. Care is needed to ensure that any media that poured on the edge of the plate is aseptically removed before motile organisms would use it as channel to contaminate the next plate
4. if there is general cleanliness of the incubator
However, if you have enough space, you can incubate an organism per time
If proper precautions are taken, there should be no problems of cross contaminations between cultures. But it would be better to grow fungi and bacteria separately and also take into account spore dispersion.
If the incubater is maintained well with proper prior sterlizations you can keep the bacteria together, no problem
Yes, since your bacterial cultures will be in the petri dishes with covers.
No, if two different bacterial culture requires different incubating temperature. You can't grow a bacterium requiring 45oC to grow e.g. Salmonella on MSRV and another bacterium requiring 35oC e.g. E. coli on TSA in the same incubation.
If properly handled, you definitely can incubate different bacterial plates (with different organisms) in the same incubator. The incubator has to be cleaned well, the plates must have their proper lid on and must be placed carefully (to avoid spilling of specimen or moisture). Also make sure not to completely fill the incubator with plates, tubes, etc...
Yes you can with proper handling. Also incubator should be often cleaned using 70% alcohol.
Precautions should be taken in cases of fast spore germinating microbes! A parafilm wrap is a best option avoid that! Also avoid coincubation of BSL-1 organism with BSL-2 & BSL-3 for safety! A proper handling and time to time fumigation may work too
A part from the fungal cultures you can incubate different types of bacterial cultures in the same incubator
Also I can say yes. Because in practice we incubate multiple plate in the same incubator and we never had contamination problem.
Wha is necessary is to dry properly the plates
As Adodo said OK,but if you have availability of other incubators.Make one for fungi and one for bacteria.
Yes correct, you should do that because the fungi usually incubated at 20-25 while the bacteria at 37
As the plates are covered by their lids and the incubator is clean, no contamination occur from nearby plates
however, it is advisable to have separate incubator for fungal plate cultures
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