What would be the concentration of a 10μl aliquot taken from a 10ml solution with a concentration of 1000mg/ml?
Hello, you need to mention final value for calculation of concentration.
If your solution concentration is 1000mg/ml so 10μl will contain 10mg of substances.
Good luck!
Since 10 uL is 0.010 mL (there are 1000 uL in every 1 mL).
Therefore, 0.010 mL x 1000 mg/mL = 1 mg
10 mg/10 mL = 1 mg/mL
Since there are 1000 uL in every mL, 10 uL is 0.010 mL, thus the mass is 0.010 mg or 10 ug.
Since the concentration of your stock solution is 1000 mg/ml , if you take an aliquot of 10 ul, the concentration of the solution remains same unless you further dilute to a certain volume. But the content in 10 ul will be 10 mg only.
An aliquot volume has the same concentration as its original solution. So it is still 1000mg/l and may change only by dilution or concentration process.
The concentration should be the same since the aliquots are not undergoing any further dilution. The principle is that any part thereof of any concentration will have an identical concentration to the original stock solution.
The concentration (w/v) remains 1000mg/ml, however the mass of solute in 10μl aliquot is 10mg. i.e 10ml solution contains 10,000 mg; 10μl = 0.010ml will contain 10,000 mg/10ml x 0.010ml =10mg.
Basically 10mg/10μl = 1000mg/1000μl = 1000mg/ml
solution is the same solution 1000mg/ml
concentration changes only when you add the aliquot into another solution.
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