20 May 2022 0 8K Report

Has anyone translated nematode identification keys found in Tom Bongers' "De Nematoden van Nederland" from Dutch to English?

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Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

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Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do you calculate the substrate concentration of an assay in enyzme kinetics given the volume and the buffer volume at a given 6mM of the enyzme?

In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...

25 October 2020 1,702 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Trace metal speciation?

I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...

18 August 2020 4,333 4 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

What is the best way to hold AFM cantilevers during piranha cleaning?

Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...

24 March 2019 8,787 1 View

Is rice bran oil good for frying?

I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...

29 May 2018 1,311 1 View

Can somebody assist me by giving me standards for fumonisins for my MSC project?

I am a Master of Biotechnology student at Kenyatta University, working on a project on determination of Fumonisin FB1, FB1, FB3 and FB4 on maize samples. I would like to use HPLC technique of...

30 October 2017 2,079 3 View

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How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

How to fetch data of a .sslist file?

We are using Mauve software for alignment of genomes of nematodes having genomic size approximately 100 Mb. One of the output file generated using this software has extension .sslist file. Unable...

24 September 2020 7,520 1 View

Could someone recommend me a journal for the publication of a short note discussing the validity of some parasitic nematode species?

These species were described in a PhD thesis and all of them but one remain unpublished.

06 June 2020 4,786 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Why second stage juveniles are used for DNA extraction from nematode for identification purpose? Can I use female or male for the same purpose?

I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...

26 March 2020 7,419 3 View

Where can I get a reliable list of Wieser's (1953) nematode feeding type classification?

Numerous articles cite it but nobody seems to provide an actual list of the taxa and which of teh four groups they are assigned to.

21 March 2020 5,990 5 View

Does anybody provides identification of avian nematodes in India?

Nematodes were found in some birds during the postmortem. I would appreciate if somebody expert in nematode taxonomy would help in the identification ...

30 January 2020 7,758 3 View

Any Nuclei extraction from Nematode that are not super labour intensive?

I am culturing 20 species of Caenorhabditis and require a technique for nuclei extraction for HiC sequencing. There are many methods out there that take from 15 minutes to a day's worth of...

17 January 2020 3,837 1 View

Please suggest the primers for the Aphelenchoides group characterization?

I have got the population of Aphelenchoide nematodes. I am trying to amplify their DNA by using different primers as given below: 1. for ITS - 18S: 59-TTGATTACGTCCCTGCCCTTT-39 (forward) and 28S:...

31 December 2019 5,593 1 View

Which statistical analysis is proper to compare more than 3 populations of nematode?

Hi I have some morphological characteristics assessed in three Ditylenchus destructor populations from different regions and I would like to know how to compare these measurements together?

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