Hello,

I want to determine infection load in CLP mice after 24 hours from blood and peritoneal lavage. In BALB/C, I have ligated 75 % cecum and punctured the cecum twice with 18 G needle , then after 24 hours collected blood and 1 ml peritoneal wash, so what dilution range of blood and peritoneal wash should I use to determine CFU on Sheep blood agar plates aerobically.Another question is, in which anticoagulant should I collect the blood for CFU determination?

Thanking you,