Pellet of single cell suspension of mouse splenocyte is not dissolving in TRI reagent even after vigorous vortexing, rather it has formed a tight mass.Earlier it used to, but this time it did not.Any reasoning for such phenomenon?
Thank you,
http://www.med.upenn.edu/kaestnerlab/documents/RNAExtraction-FunctionalGenomicsCoreStandardMethods.pdf
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