Are PBMC isolated from blood collected in ACD blood bag culturable?

27 March 2017 1 7K Report

I just heard from someone that PBMC isolated from blood collected in ACD blood bag can not be cultured, is it true?

I don't believe so. Similar to EDTA, and sodium heparin, the acid–citrate–dextrose (ACD) is a commonly used anti-coagulants for collecting blood samples. I donot see the reason why the cells cannot be cultured in following assays.

Please find the attached publication which addressed that these anti-coagulants did not differ in terms of cell recovery, viability and function (as assessed by IFN-γ ELISPOT and intracellular staining of PBMCs stimulated with CD8+ T cell peptide epitopes).

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010910/

Hope this helps.

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