I find that starting with a midline incision of the skin and afterward the peritoneum produces little to no blood loss. If you have tried this, make sure the animal is not too warm as well to limit peripheral vasodilation.

Good luck

After opening the skin the peritone is tented in the midline by holding it on both sides by two non-toothed forceps, then incising  it with with knife to form a hole in the peritoneal cavity through which incision is compelted up and down under vision using scissor thus avoiding injury of intranal  organs and vessels

Hello Komal, I conducted a protocol using mice to produce adhesions and later evaluate the effect of vitamin A and NSAID to diminish them. Of course if you deviate from the midline and get to muscle you may get important bleeding, but regarding peritoneum  always make a small incision with scissors making traction between two clamps (may be Halsted´s) and transiluminate with lamp ligth in order to avoid cutting on a blood vessel . Of course when we operate on humans we use energy devices like ligasure or the basic monopolar electrosurgery unit but in experimental surgery in animals we dont have those available

Greetings