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Questions related from Khan Md Taufiqul Arif
I have been trying to design primers for microRNA expression. The image shows that there are 2 sets of forward primer (Tm calculated in two different manners) and one universal "reverse primer" as...
11 November 2018 5,287 0 View
I have a set of breast cancer cell lines and will use HMEC as a control population. Since HMECs have the tendency to show unusual characteristics along with the passages so I would like to know...
08 August 2018 6,704 0 View
I have found a SNP in a microRNA gene from my association study and now want to do the expression analysis in breast cancer cell lines (sub-type specific). What are the factors should I consider...
05 May 2018 741 0 View
I have found a miR-SNP which is significantly associated with my breast cancer case-control cohort. Now I want to see its expression in different breast cancer cell lines to find out the best...
04 April 2018 5,217 4 View
I have got .bed .bim and .fam files from a database and need to see whats inside. But I don't know the command to do so. As well as I would like to view my own .ped and .map files in the command...
09 September 2017 8,835 0 View
The drug compound (a multi tyrosine kinase inhibitor) has been showing significant anti-inflammatory activity. Planning to test if it has anti-arthritic activity. I would like start with some in...
10 October 2016 9,335 4 View
I have used the drug as a broad term here. Specifically my focus is on those drugs which have antioxidant property. Is there really any time point at which cells will respond best to the...
08 August 2016 4,926 0 View
Previously I tried with EBM-2 (cell: HUVEC), but I found that in cell free condition EBM-2 in presence on DCFDA (20uM) gives very high fluorescence. My plan is to replace EBM-2 with HBSS. But, I...
07 July 2016 1,266 5 View
To get the best ROS generation (i am expecting 40-50% increase than that of control), how long my HUVEC should be starved and what would be the serum percentage? i am using EBM-2 (Lonza) without...
07 July 2016 9,363 5 View
I am culturing HUVEC up to 80% confluency (EGM-2; Lonza), then let them serum starved (EBM-2; Lonza) for 24hr and treated with TNF-a (10ng/ml) for 30min. With DCFDA I didn't get that much of...
06 June 2016 2,071 6 View
Trying to measure protein concentration for Lucigenin assay. But even culturing at more than 80% confluency, after centrifugation and sonication getting only 330ug/ml of protein. Hard to maintain...
06 June 2016 1,331 2 View
I am not controlling any of these factors, but feeling a bit shaky. Do I need to maintain a certain wavelength and what would be the optimum temperature for that? Thanks in advance.
06 June 2016 185 5 View
i would like to test my compound in vitro. which cell line should I use to generate atherosclerosis? to prove my compound's (Kinase inhibitor) potency which assays would be rational ?
05 May 2016 546 4 View
need to apply mAb against DDR1 and DDR2 to block their action in vitro. My plan is to apply the mAbs directly to the cells in well. But confused, whether the typical antibodies can block their...
05 May 2016 6,311 3 View
is there any optimum time frame for that?
05 May 2016 8,182 5 View
HUVECs are being starved for 24 hr (in 96-well) and same volume to of DCFDA is being added directly. Do I need to replace the media before adding DCFDA? Can cytokines cause any effect on ROS...
04 April 2016 3,534 2 View
need to inactivate integrin a2b1. HUVEC in collagen-I coated well is producing excessive ROS than that of non-coated wells. need to know why... thus need to block the particular integrin...
04 April 2016 5,487 2 View
need to inhibit integrins but nor sure about the RGD peptides are potential enough. Which commercial RGD peptide would be better? i'll observe if inhibition of integrin signaling can reduce ROS...
04 April 2016 9,818 11 View
Best mouse strain for sepsis model. Is there any background for selecting the appropriate strain?
03 March 2016 9,387 2 View
Found many articles that there is always a pre-treatment before injecting the LPS (in mouse), but how can this data be interpreted considering the real status of a patient, who can't have a...
03 March 2016 9,038 2 View
Need to know the concentration, dosage and preparation of LPS, I am going to test my drug whether it has any anti-sepsis action.
02 February 2016 9,533 4 View
Assay: Cytokine profile and Survival rate determination Animal Model: C57BL/6 mouse (6weeks) LPS: E. coli: O111:B4, O55:85, O127:B8, Salmonella enterica ser. enteritidis/typhimurium Dose: 15mg/kg...
02 February 2016 7,456 3 View
LPS mediated sepsis in mice for cytokine profile to test a drug's anti-inflammatory activity.
02 February 2016 1,400 3 View
HEK293/DDR2 showed a little increase in ROS production in comparison to HEK293 cell line, but ROS generation was found to be decreasing in HT1080 (HA-DDR2) cell lines in contrast to HT1080 cell...
01 January 2016 1,225 3 View
I have found that a tetrameric form of Ang-I would do good but, what is the molecular weight of that particular state and to which company I should look for.
01 January 2016 1,281 2 View
I have been trying to develop a ROS profile of HEK/DDR2 cell line. Within the 4hr incubation span, after 3hr I exposed cells to UV-B for 20 min and then again incubate for 1hr. But my...
12 December 2015 850 1 View
I have been searching for SNPs in miRNA genes (formulated panel of 65) in a designated breast cancer population by iPLEX/MALDI-TOF-MS. But after that just got stuck into and couldn't go...
01 January 1970 5,280 1 View
Dear Tuan Zea Tan, My research is focused on association of miRNAs to the molecular subtypes of breast cancer and their clinical significance. I'd really appreciate if you could share the...
01 January 1970 6,687 2 View
