19 Questions 28 Answers 0 Followers
Questions related from Justin Tan
Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....
24 November 2020 7,289 20 View
Dear all, Recently i have been trying to measure colour difference between my samples. I am a little confused as to why i cannot just use the basic colours - i.e., red, green and blue to measure...
03 August 2020 7,531 2 View
Dear all, recently i am working on chromametric measurements and i am keen to know if Image J / Fiji is able to help me determine the TCD (total colour difference) of my samples. To do so, i will...
05 May 2020 7,264 2 View
Dear all, i am currently working on 4D printing of gelatin. I haven't seen any work using gelatin in 4D printing as a shape memory polymer. Kindly share with me some publications which has or from...
19 March 2020 10,102 3 View
Dear all, recently i have tried to prepare a composite gel containing gelatin with transglutaminase. After placing the mix inside the incubator, the entire gel turned white. Why is this...
12 March 2020 2,949 4 View
Dear all, i am currently working on prolong heated gelatin (heated at 65 degree celsius in the heat oven for 7 days) and i would like to know if there are any known benefits/applications to...
12 March 2020 7,641 0 View
Dear all, Recently i ran an oscillation test using gelatin mixed with transglutaminase (conditions were 40 degree celsius, 1 Hz oscillation frequency and 1% oscillation strain) and i realized...
11 February 2020 994 3 View
Dear all, good day! I have a question regarding the use of NaOH for lysis of mammalian cells. Is it a common practice? Are there any publications which have used NaOH to perform cell lysis? If...
09 April 2019 5,936 3 View
Dear all, I am trying to create co-culture systems using different cell types. I have realized that for some cell types, when mixed together, do not produce any aggregations or polarizations,...
17 December 2018 802 2 View
Dear all, my question is as above for immunofluoroscence staining purposes. Thank you for your kind responses.
10 October 2017 4,890 2 View
Dear all, i was confounded by a phenomenon i witnessed under my light microscope - that is, when i tried mixing two different cell types together, i started to observe dark aggregates forming over...
04 October 2017 3,543 4 View
Dear all, I would like to optimise the concentration (%) of BSA to use for diluting my primary and secondary antibodies (in 1x PBS) to be used for immunofluorescence staining (and subsequently...
12 September 2017 8,500 6 View
Dear all, i am extremely confounded by what are the "best" dilutions for making stock solutions of primary and secondary antibodies to be used for immunofluorescence assay. The concentrations of...
12 September 2017 2,527 4 View
Dear all, i am keen to purchase some serum as blocking agent for pre-treatment of my samples before the addition of my primary antibodies. However, my primary antibodies are from two sources -...
12 September 2017 9,534 1 View
Dear all, Recently i tried to stain some co-cultured cells and realised that both the co-cultured cells and the mono-cell type both showed the fluorescence - indicating the presence of the...
05 September 2017 5,051 10 View
I have two stains I would like to use - namely, phalloidin, for staining the actin filaments and also DAPI for staining their nucleus. However, i cannot really find any protocols for which uses...
01 April 2016 1,780 4 View
Dear all, I am running an experiment which requires me to isolate the ethyl acetate as the organic layer in a liquid-liquid extraction, after which I would need to use the ethyl acetate layer for...
13 March 2016 3,423 6 View
Dear all, I have been trying to find the best protocol for isolating a compound (which is a product of an enzyme reaction) from the cells after incubating with the corresponding substrate. Looking...
11 March 2016 10,002 1 View
Dear all, I have been working to troubleshoot my LC-MS/MS/MS experiments for several months already. There seems to be multiple peaks of the same product ion observed on the LC-MS chromatogram but...
11 March 2016 5,578 13 View
