What are the pros and cons of the CaptureSelect ™ FcXP Affinity Matrix in antibody isolation compared to protein A and/or protein G matrix? Are there any differences based on affinity and interaction mechanisms?
Thanks in advance...
hi
It is clear from the company's advertisements that this type of column is specifically suitable for purifying recombinant human antibodies or human antibodies in culture medium containing FBS. In this case, your antibody is more pure and active than the Protein A/G column. in protein A,G or A/G elution was occurred in harsh mode ( Glycin-Hcl pH=2.5).if you need a dedicated column to isolate human antibodies and the purity and activity of that antibody is important to you, it is best to use this column. But if you are looking for a general column for antibody purification,protein A/G column is a good option.
Features of this affinity matrix include: • Mild elution, making it suitable for Fc fusion proteins • Human-specific, no binding to bovine antibodies • Excellent scalability • Non-animal derived
good luck
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