Why do we need to prepare the oxime derivates (hydroxylamine.HCL catalyzed) of carbohydrates and organic acids prior to silyl (TMS/hexamethyldisilazane) application for GC analysis? What is the principle behind the reaction and why organic base-catalyzed direct derivatization using, silyl agents doesn't work?

Could you suggest any more practical, high yielded, and direct derivatization protocol for polar compounds (especially for sugars and organic acids) profiling at GC-MS?

Thanks in advance...

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