How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

How to synthesize pyrrolium ionic salt?

I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...

30 December 2020 2,778 3 View

How can large volume ( 100 liters or more ) of carbon dots solution be purified and sieved cost-effectively?

Articles describing the use of carbon dots in agriculture have been increasing recently. Again, studies have found that carbon dots size and shape and some other features are effective. Therefore,...

30 December 2020 1,139 3 View

How to Modeling and simulation for launch vehicle?

Modeling and simulation for launch vehicle Title of graduation project for a master's thesis in mechanical engineering, specializing in Control Search assistance required? Thank you

28 December 2020 9,000 1 View

How to calculate average habitat suitability change between 2 different SDM/ENM?

Hi guys, I would love to know how I could go about solving the above question. I currently have 2 different species distribution models, one created with the current climate projection and the...

14 December 2020 864 8 View

What are good M.Sc thesis topic suggestions in construction management (specially BIM & Waste management area)?

My prefered area is in Waste management & BIM , where I have read about using 4D BIM & 5D in waste management , but I have not reached a significant research gap to work on it, so is...

03 December 2020 1,294 2 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

Please, how can I measure the value of the inductance, parasitic series resistance, and the quality factor of this structure using the ADS ?

Dear colleagues. I would like to design the floating active inductor shown in the figure below. Please, how can I measure the value of the inductance, parasitic series resistance, and the quality...

10 October 2020 9,488 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View