Islam Saadeldin

6 Questions 20 Answers 0 Followers

Questions related from Islam Saadeldin

Any idea about the replacement of SmartPak DQ3 Purification Pack, Millipore?

Our Millipore Direct-Q 3 machine is broken down. The water leaks without holding in the purification pack. The Purification Pack was never replaced since its installation in 2013. Is the...

10 November 2020 3,555 4 View

What are the acceptable biological and technical replicates in realtime RT-qPCR?

What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...

07 August 2018 5,829 4 View

How to detect trehalose in cultured cells?

Is there a method to detect trehalose in mammalian cell culture?

11 March 2018 5,258 3 View

14-day embryo rule, why not to be extended?

It is known that embryos can survive for a limited age in vitro and cannot proceed to organogenesis because lack of placentation and unlimited factors in the uterine and maternal environment. Two...

28 June 2017 3,344 5 View

What is the specificity of ELISA in evaluating melatonin from different sources or different species?

Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?

29 August 2016 5,732 4 View

What is the scientific importance of chimera/chimeric animals?

Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...

15 November 2014 8,976 10 View