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Questions related from Islam Saadeldin
Dear Colleagues, For Exosomes/Extracellular vesicles (EVs) labelling by PKH26 stain, and studying their cellular uptake. Is there anyone who tried to mix conditioned medium with PKH26 to stain...
09 November 2021 9,029 3 View
Our Millipore Direct-Q 3 machine is broken down. The water leaks without holding in the purification pack. The Purification Pack was never replaced since its installation in 2013. Is the...
10 November 2020 3,639 4 View
During the process of oocyte maturation, asymmetric divisions result in segregation of the maternal genome and highly asymmetric partition of the cytoplasm, which generates the small polar body...
01 October 2019 3,107 9 View
What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...
07 August 2018 5,917 4 View
Is there a method to detect trehalose in mammalian cell culture?
11 March 2018 5,333 3 View
Dear Colleagues, During in vitro maturation of "camel" cumulus-oocyte complexes, I frequently noticed that cumulus cells start to attach to IVF non-treated dishes within few hours of culture. I...
11 October 2017 8,658 8 View
It is known that embryos can survive for a limited age in vitro and cannot proceed to organogenesis because lack of placentation and unlimited factors in the uterine and maternal environment. Two...
28 June 2017 3,440 5 View
Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?
29 August 2016 5,802 4 View
I am looking for a recommended method for cryopreservation of Tilapia milt and eggs with high fertilization rate. The resulted fresh fertilized eggs will be mincroinjected with DNA...
31 August 2015 1,313 7 View
I have a cloning vector (fig. a) with target gene bMPRO. I want to insert this target gene into the attached vector (fig. b) for using for cell transfection. How can I solve this problem? any...
18 May 2015 383 5 View
Most articles describe that insulin increases the potassium uptake by the cells. And the mechanism is totally independent on glucose uptake. Also, we assume that insulin enhances the Na/K pump,...
29 November 2014 9,475 6 View
Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...
15 November 2014 9,071 10 View
The last update of haploidization and its future perspectives was written by Drs Jan Tesarik and Carmen Mendoza in 2002 (Somatic cell haploidization: an update, Reproductive BioMedicine Online,...
12 May 2014 8,698 7 View
Today 24 April 2014, Bianchi et al. 2014 (Nature 508, 483–487, doi:10.1038/nature13203) reported that oocyte's "Juno" is the reciprocal receptor of sperm's "Izumo1". Their binding is essential...
24 April 2014 2,172 2 View
I treated the mature cumulus-free oocytes with pronase 0.3% (w/v in PBS), the zona pellucida was digested within 60-90 seconds however when the oocytes were first electrically activated...
31 March 2014 4,818 14 View
I need a simple protocol for karyotyping of preimplantation embryos (morulae and blastocysts).
09 March 2014 10,014 14 View
I observed cumulus-oocyte complex circled anti-clockwise during bovine IVF. I can not find any scientific reason for this behavior.
13 June 2013 229 13 View
Primary cell culture of buffaloes
09 December 2012 2,195 3 View
