As long as my experience, cryoprotectant that gives a good viability is ethylene glycol with slow freezing (at least 1 h equilibration at 4C). It will better to remove all semen plasma by centrifugation before loaded by Tris or other buffer and added an antioxidant.

I don't have any experience about Egg freezing.

Thanks Erif, could you please explain the appropriate concentrations.

Dear professor

I am inviting you to read this 2 papers  

Effect of butylated hydroxytoluene (BHT) on the cryopreservation of common carp (Cyprinus carpio) spermatozoa

F. Ö˘gretmena,∗, B.E.˙Inanan

Mugla Sıtkı Kocman University, Faculty of Fisheries Department of Aquaculture, 48000 Mugla, Turkey

Mugla Sıtkı Kocman University, Faculty of Science, Department of Biology, 48000 Mugla, Turkeya

Or

Effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) sperm

Cengiz Yildiz , Ilker Yavas , Yusuf Bozkurt , Melih Aksoy

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Mustafa Kemal, Hatay, Turkey

Department of Aquaculture, Faculty of Marine Science and Technology, University of Mustafa Kemal, Hatay, Turkey

Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydın, Turkey

good luck and best regards

Thanks Mr Larbi for the information.

Dear Ghazal, 

Thank you for your answer.

Dear Islam,

I have been working on sturgeon sperm cryopreservation for a long time. I recommend 2 following papers which I think will be what exactly you need.

Good luck

Shahrouz

Thank you so much Dr Noveiri  for your answer, it is really helpful.

Regards,

Islam