Hello

I'm not sure what kind of cells we want to use but check out this article.

Membrane transporters and cytoplasmatic pH regulation on bovine Sertoli cells.

P F Oliveira, M Sousa, A Barros, T Moura, A Rebelo da Costa. The Journal of membrane biology. 01/2009; 227(1):49-55.

https://www.researchgate.net/publication/23563990_Membrane_transporters_and_cytoplasmatic_pH_regulation_on_bovine_Sertoli_cells

I've done primary cell cultures of Sertoli cells from adult bulls. It might help you...

Good luck

Article Membrane Transporters and Cytoplasmatic pH Regulation on Bov...

Thank you Dr Oliveira for your reply. The cell of interest is the "buffalo fibroblast". My previous work was in the "Cow" but now I am working in the "Buffalo" however the principle is the same, I am wondering if there is something special for the buffalo.

I have done lot of work on primary cell culture in buffaloes, I have work both on buffaloes and cattle, Their is nothing change between two species, except for buffaloes you need to maintain CO2 incubator temp to 38.5C.