Please read this articles.... "Chromosomal analysis of embryos produced by artificially inseminated superovulated cattle"

WA King, Verini Supplizi A, HEP Diop, D Bousquet

Genetics Selection Evolution. 01/1995; DOI:10.1186/1297-9686-27-2-189

Thank you Naresh for sharing this paper. This study showed a brief note for embryos of 2-4-8 cell stages, still I need a detailed protocol for karyotyping of morula and blastocyst.

[email protected]

Thanks

Thank you very much Dr Gupta.

Thanks Federica.

 Hi Islam, although it's an old post, I'm still trying my luck to ask you if you have a working protocol for Karyotyping of mouse blastocysts now. Also, are there any changes in Karyotyping different stages of embryos? It would be great if you could share your experience. Thank you. 

Hi Pooja!

I didn't use mouse embryos before but as far as I know, the protocol of karyotyping (from oocyte till blastocysts) is similar to most of the mammalian embryos.

Try to find this useful book; 

http://www.cshlpress.com/pdf/sample/2013/ManipMouse4/MME4FM.pdf

Good luck.

Thank you for your reply Islam. I think the protocols I have so far come across, are very similar to karyotyping any cell type, the only tricky part being the embryo splitting procedure. Could you perhaps share your protocol and any tips if at all? Thanks again. Regards.

There is no need for embryo splitting if you analyze the whole embryo. But if you want to analyze some cells, you may take biopsy either through microdissection or embryo splitting or through micromanipulator, and both need training for several times.  

Dear Islam, please share the protocol for karyotyping, I want to perform in 2-cell to 8-cell stages. Thank you so much. E-mail: [email protected]

Thanks Jun-Xue Jin

I sent the e.mail to you.

Regards

Dear Dr. Islam, please share the protocol for karyotyping, I want to perform in slow cleaving embryos. Thankyou E-mail: [email protected]

Assalamualaikum islam, please share protocol for embrio karyotyping to my email [email protected]