Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it. Please i need a step by step guide, I will be grateful for your response .
Please, first ask your supervisor who assigned this project to you and/or read some molecular biology text books. The RG community will certainly be happy to answer specific questions but posting more or less standard procedures is not its primary scope,
@ Sabbine Strehl, I have actually done this, I wanted to checkmate if the steps I followed was right , and If it is what should be the transfection efficiency