Proteosomal degradation ubiquitin dependant or ubiquitin independent?

01 January 1970 1 2K Report

How can I determine if the protein in my diseased cells is degraded by the ubiquitin dependant proteasome or by the ubiquitin independent proteasome

Albert Lee

as a quick and dirty try blocking the proteasome (MG132) or autophagy (chloroquine) and see if your protein accumulates (e.g. by western blot) or has higher laddering (respresentative of ubiquitin linkages)

Badges
Science topic

More Gehan Abdelaziz's questions See All

Comment publier un travail sur la plateforme ?

Publier des documents scientifiques

26 June 2024 7,343 1 View

Can people help me complete my questionnaire?

I am conducting research with title "Cost Overrun and Time Delay Prediction Using Machine Learning Algorithm: Application of a Hybrid Intelligence Model." and I need to collect responses on...

23 February 2024 3,544 1 View

Which is the essential book for learning more about singular elliptic and parabolic equations?

Dear Colleagues, On follow up on our new interest. Nowadays, research in singular elliptic and parabolic equations spans various areas of mathematics and applied sciences, including mathematical...

13 February 2024 5,088 0 View

How can I to use magnitic field in ansys fluent ?

I hope to get a clear answer on this matter

14 November 2023 2,926 4 View

Combined effect of both rotary inertia parameter (r_g/L) and shear coefficient in bending frequencies?

The shear correction factor (k) and the rotary inertia parameter (r_g/L) are both known to reduce the bending frequencies of Timoshenko beams. However, the effect of increasing each parameter on...

04 September 2023 9,195 0 View

I am trying to get Ionosonde Ne by using SAOExplorer, but it requires username and password?

I sent an email to the circled mail in the following screenshot without any reply. Is there any other way to connect to this website?

07 August 2023 5,527 0 View

What is the importance of applied mathematics in the field of industry?

importance of applied mathematics

25 July 2023 626 9 View

I am trying to read all the electron density profiles for one day by this code, but the result file is empty. can any one help me solve my problem ?

clc;clear; % Create an empty array to store the electron density data ne_array = []; % Loop through the files in the folder folder_path = 'D:\ionPrf_prov1_2020_002'; % replace with the path to...

02 July 2023 1,401 1 View

What can be the latest research gaps that need to be covered in 3D printing in construction field?

Kindly mention you points.

07 June 2023 794 3 View

Where can I find the answers for the word associates test (Read, 1993,1998) and the productive levels test (Laufer & Nation, 1999)?

Hello dear vocabulary acquisition researchers, I am looking for the answer sheet of the vocabulary depth test (Word associates Test) and the productive vocabulary levels test (Laufer & Nation,...

12 January 2023 8,247 4 View

Why p53 is not higher kda in westernblot after ubiquitin IP?

Dear All, I use HEK293-T cells for preperation. I treat them 24h with epoxomicin. And then I isolate proteins using RIPA buffer. I precipitate overnight 5 mg protein with 25 micro liter of...

29 March 2024 3,436 0 View

Western blot membrane conservation ?

Hello¸ Im PhD Student in UQTR ( Canada), I have a crucial experience for my project thesis : I have a WB (western blot) membrane that I need to store for a long time for incubation with other...

25 February 2024 7,082 1 View

Hi, can anyone suggest anti-ubiquitin antibody that is specific for mono-ubiquitination and does detect not poly-ubiquitination or free Ubiquitin?

In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.

19 November 2023 7,374 0 View

How to increase the efficiency of targeted protein degradation during cancer chemotherapy?

In recent years, various strategies for targeted protein degradation have been developed using the ubiquitin-proteasome system or the autophagy-lysosome system (PROTAC, LYTAC, etc.). However,...

22 September 2023 6,585 0 View

How to clone a potentially toxic insert for rice transformation?

Hello fellow scientists! I'm currently trying to construct a vector for agrobacterium-mediated transformation into rice. Our construct aims to overexpress two proteins (protein A and protein B),...

14 September 2023 6,194 3 View

For confocal microscopy, is there a way to tag ubiquitin protein without hampering its job in vivo?

Hello everyone! I am planning an experiment where I would like to check ubiquitin transfer using confocal microscopy; thus, I intend to add a fluorophore tag to the ubiquitin molecule. I read a...

12 August 2023 7,532 1 View

Will survivorship bias invalidate ubiquitination blotting?

I'm interested if a protein is being degraded. My idea is to western blot my samples, add an antibody for ubiquitin, image, wash off the antibodies, add an antibody for the protein of interest,...

04 August 2023 2,977 2 View

Commercially available antibody to detect yeast proteasome subunit?

I am wondering which are the antibody commercially available to detect specifically the proteasome subunits in budding yeast (Saccharomyces cerevisiae). Any suggestions and recommendations really...

17 July 2023 5,182 0 View

Is one Ubiquitin sequence enough to induce the degradation of recombinant protein?

I want to express a recombinant protein with a Ubiquitin tag at the N-terminus to induce its degradation via the proteasome. Would one ubiquitin sequence be enough or should I add more than one in...

24 May 2023 2,805 1 View

How can I get 'phosphoribosyl ubiquitin(PR-UB)' from ubiquitin(UB)?

Now ,I can get ubiquitin(UB) by Vector pQE-30.How can I get phosphoribosyl ubiquitin(PR-UB)? Need protocol, plz, thanks.

27 February 2023 6,841 0 View