Hello everyone,
I'm studying cell cycle regulation in diseased MSC,I did BrdU cell cycle analysis,and there was significant delay in G1/S phase in diseased cells in comparison to normal cells
I want to analyze gene and protein expression of cell cycle regulatory factors
My question is how to make sure that all the cells at G1/S phase transition ?
Dear Gehan Abdelaziz,
In order for all (most except for cells in G0) cells to be on the G1-S border, double synchronization must be done. With aphidicolin or a combination of serum starvation and aphidicolin.
You can read more about this in my review. Uzbekov, 2004
Biochemistry (Moscow),
Analysis of the Cell Cycle and a Method Employing Synchronized Cells for Study of Protein Expression at Various Stages of the Cell Cycle
The full text in English is in ResearchGate.
Article Analysis of the Cell Cycle and a Method Employing Synchroniz...
Rustem E Uzbekov
Best wishes,
Rustem Uzbekov
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