What is better for quantifying protein levels, ELISA or flow cytometry?

What are the advantages/disadvantages for each in quantifying intracellular protein levels?

05 June 2018 9,883 2 View

What assays can be used to detect cell cycle arrest in culture?

Preferably assays involving flow cytometry. Thanks.

05 June 2018 9,677 3 View

Best protocol to use for protein level detection?

There are many methods being used in the industry to measure protein levels: from Western Blots, to ELISA, to RT-PCR, to immunoblot, to flow cytometry. Could I have a clarification on the contexts...

04 May 2018 962 3 View

Is Capan-2 cell line a form of pancreatic ductal adenocarcinoma?

For anyone who has worked with this cell line before, could you please confirm if Capan-2 is a format of pancreatic ductal adenocarcinoma? Thanks.

03 April 2018 927 2 View

Cell seeding density for Senescence-Associated Beta Galactosidase Assay?

Hi all, Does anyone know if there has to be a specified seeding density for the senescence-associated beta galactosidase assay performed in a 96-well plate? Or does any seeding density generally...

03 April 2018 9,638 1 View

Can p53 Activation induce more replicative senescence than apoptosis?

Hello all, I recently treated telomerase-inhibited A549 cells with 5 uM RITA, a p53 activator drug, and measured cell viability using the MTT assay. While wild type A549 experienced an expected...

02 March 2018 3,019 4 View

Proper PCR DNA Polymerase to Use For DNA seqeuncing?

Hi all, When I am conducting PCR in order to amplify a certain DNA segment for subsequent Sanger sequencing, would using the conventional DreamTaq Green Mix from Thermo Fisher work fine? Or...

01 February 2018 9,140 3 View

Assays to perform for measuring replicative senescence?

Hello all, I am performing telomerase inhibition experiments that will result in replicative senescence in cancer cell culture. Can anyone recommend assays to use to measure replicative...

01 February 2018 4,206 2 View

Why must cancer xenografts occur in immunodeficient mice?

Hello, I'm curious as to why cancer xenografts must occur in immunodeficient mice. Ordinarily would the mouse immune system eliminate the cancer cells? And why would this not occur in regular...

31 December 2017 1,456 5 View

Why did A549 Cell Viability Increase upon treatment with p53 activator drug RITA?

I treated wild type A549 cells with 10 nM RITA, a p53 activator drug, and the cell viability increased 18.7% in a statistically significant manner (p

31 December 2017 1,160 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View