Did anyone used Abs to block CD80/CD86 on APCs in vitro?

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Why does an architect change his/her style?

I am writing an article on a Turkish architect. The style of his implementations changed in time from modernism to post-modernism. Do you happen to have written articles on change in style? Do...

03 January 2024 7,210 0 View

How potential is AI for Aestetics in Architectural Education?

Beauty in architecture is defined in relation with style and type. How AI contributes to that?

28 November 2023 926 2 View

How can I preserve samples for DNA extraction and methylation analysis?

I want to preserve tissue samples to use them in DNA extraction for epigenetic and methylation analysis. How can I preserve samples for DNA extraction and methylation analysis?

21 November 2023 5,108 0 View

A Neuroscientific explanation for differences in positive emotions but not in negative emotions?

Hi everyone! I recently conducted a research study investigating the impact of virtual reality (VR) on learning and memory of difficult knowledge. The results I obtained from administering the...

28 May 2023 5,101 2 View

Has anyone tried reverse transcription from a DNA template?

Has anyone tried reverse transcription from a DNA template? How well does it work compared to an RNA template?

28 March 2022 8,708 4 View

Is nested t-test the best way to analyze a small sample with many values?

Hello everyone, I have a question regarding a statistic test. I will give you the background first. I am testing different characteristics of microglial cells. I am testing the difference between...

22 March 2022 4,152 3 View

Has anyone had experience staining fixed human brain tissue?

I have brain tissues that are fixated for a few years and I plan on staining them using fluorescent Neu-N antibody. I know that it usually does not work without antigen retrieval. I could not find...

24 March 2021 6,692 3 View

Why my gene is upregulated following an siRNA?

I am using Taqman to detect a transcript following a knockdown experiment of that gene in cells using esiRNA from Sigma (a mix of 19 siRNA sequences targeting different areas on the transcript of...

14 March 2021 3,233 3 View

What is the RNA concentration in cell culture media?

The RNA concentration in serum according to literature is about 5-40 ng/ml. However, there is very little information about the RNA concentration in conditioned medium of cultured cells....

27 June 2019 6,501 7 View

Does MHC-II Ab clone M5/114.15.2 block the receptor?

Hi, I want to sort cells using clone M5/114.15.2 (MHC class II), and then use them in a T cell activation assay. Does anyone know if the bound antibody from sorting will block the MHC-II receptor...

10 April 2018 1,558 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to do Mann-Whitney U test with Bonferroni corrected p-values?

Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...

31 July 2024 6,464 5 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Bonferroni correction. I have independent t-test, paired t-test and ancova conducted. Which test would require Bonferroni adjustment?

I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...

28 July 2024 7,827 6 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View